Composite

Part:BBa_K3868096

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 14:33, 16 October 2021 by Registry (Talk | contribs)

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pTarget-8G

pTarget-8G plasmids contains pj23119 and sgRNA-8G etc, which can target the RBS sequence of T7RNAP for Cas9 expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 188
    Illegal SpeI site found at 73
    Illegal PstI site found at 200
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 182
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 50
    Illegal SpeI site found at 73
    Illegal PstI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 182
    Illegal BglII site found at 212
    Illegal XhoI site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 188
    Illegal SpeI site found at 73
    Illegal PstI site found at 200
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 188
    Illegal SpeI site found at 73
    Illegal PstI site found at 200
  • 1000
    COMPATIBLE WITH RFC[1000]


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