Coding
Part:BBa_K3745002:Design
Designed by: Jiaqi Tang Group: iGEM21_BNDS_China (2021-09-29)
rhlB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 247
Illegal AgeI site found at 859 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
na
Source
To facilitate genetic engineering, the endogenous enzymes rhlB is transformed from P.Aeruginosa into E. coli because E. coli is a model organism and is well-researched.
References
Du, J., Zhang, A., Hao, J., & Wang, J. (2017). Biosynthesis of di-rhamnolipids and variations of congeners composition in genetically-engineered Escherichia coli. Biotechnology Letters, 39(7), 1041–1048. https://doi.org/10.1007/s10529-017-2333-2
Gong ZJ, Peng YF, Zhang YT, et al. Construction and optimization of Escherichia coli for producing rhamnolipid biosurfactant. Chin J Biotech, 2015, 31(7): 1050–1062.