Composite
Part:BBa_K3763003
Designed by: Liran Mao Group: iGEM21_WHU-China (2021-10-18)
pFadD promoter with LacI repressor regulating downstream GFP with RBS BBa_K3763000
123
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 30
Illegal suffix found in sequence at 230 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 30
Illegal SpeI site found at 231
Illegal PstI site found at 245
Illegal NotI site found at 36
Illegal NotI site found at 238 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 30
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 30
Illegal suffix found in sequence at 231 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 30
Illegal XbaI site found at 45
Illegal SpeI site found at 231
Illegal PstI site found at 245 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 938
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Categories
Parameters
None |