Composite

Part:BBa_K3790228:Design

Designed by: Chongwen Cao   Group: iGEM21_Fudan   (2021-10-17)
Revision as of 21:56, 18 October 2021 by Wave (Talk | contribs)


GFP controlled by σS promoter fic_improved


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


Design Notes

The following primers were used for constructing this part. 

f_pET_dps1	5-GATGTCTGCCTGTTCATCCG
f_pET_dps2	5-CACATAGCCGGTGCTATACTTAATCTCGGAATTGTGAGCGGATAACAATTCC
f_pET_GFP	5-GATATACCATGGCTGAGGGATGCGTAAAGGAGAAGAACTTTTCAC
r_pET_dps1	5-GTATAGCACCGGCTATGTGTTCCGCTATTCATTTCGCGGGATCGAGATC
r_pET_dps2	5-CGGATGAACAGGCAGACATC
r_pET_GFP	5-CAGCCGGATCCTTATTTCGCTTATTTGTATAGTTCATCCATGCCATGTGT
f_pBAD_dps1	5-CAGCTGTCCCTCCTGTTCAG
f_pBAD_dps2	5-CACCGGCTATGTGTTCCGCTATTCggatccgctaatcttatggataaaaatgc
f_pBAD_GFP	5-ggtgagccagtgtgaATGCATGCGTAAAGGAGAAGAACTTTTCAC
f_pBADtran_tem	5-CAGACATtttttttacctccttaactctagtagctag
r_pBAD_dps1	5-GCGGAACACATAGCCGGTGCTATACTTAATCTCcatacccgtttttttgggctagctac
r_pBAD_dps2	5-CTGAACAGGAGGGACAGCTG
r_pBAD_GFP	5-ggaggtaaaaaaaATGTCTGTTATTTGTATAGTTCATCCATGCCATGTGT
r_pBADtran_tem	5-GCATtcacactggctcaccttc



Source

References