Coding
Part:BBa_K3799000
Designed by: Shubhamay Das Group: iGEM21_IISER_Kolkata (2021-09-30)
EiCsm6,A CRISPR system endoribonuclease
This part encodes Csm6, a type III-A CRISPR-associated protein from Enterococcus italicus that degrades cyclic hexa-adenylate activator using both CARF and HEPN domains .cyclic hexa-AMP recognition by the CARF domain activates the Csm6 HEPN domains for collateral RNA degradation.It protects bacteria against transformation with plasmids containing DNA homologous to its spacer regions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 314
Illegal XhoI site found at 367
Illegal XhoI site found at 1054 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//awards/basic_part
//cds
//function/crispr
//cds
//function/crispr
chassis | E. Coli |