Part:BBa_K2656003

Promoter HSL-mediated luxR

Figure 1. DNA basic parts domestication. First construction refers to promoter GB domestication.

Part BBa_K2656003 is the promoter BBa_R0062 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. This promoter is positively regulated by LuxR together with Acyl-homoserine-lactone. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the Golden Gate assembly protocol .

Ecuador 2021's characterization

We characterized this promoter using the composite part K3893028 that we built by assembling together

• BBa_K2656122: a Level 1 transcriptional unit expresing GFP under the control of the pux promoter (Golden Braid compatible)
• BBa_K2656114: a Level 1 transcriptional unit constitutively expressing luxR gene (Golden Braid compatible)

Device K3893028 allows us to measure GFP expression levels for different AHL induction concentrations.

Figure 1. SBOL Visual representation of the device K3893028 from Ecuador 2021 Team

Following a standard experimental procedure and the IGEM 2018 Interlab calibration for Particles and MEFL we obtained the temporal behavior of the device for different inducer concentrations.

Experimental reuslts for characterization of pLux promoter.

Final GFP expression levels for different AHL inducer concentrations.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]