Signalling

Part:BBa_K4061070

Designed by: Diya Agrawal   Group: iGEM21_HKUST   (2021-10-14)
Revision as of 07:40, 14 October 2021 by Registry (Talk | contribs)

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pOmpF and pOmpC with reporters (TCS)

The two component system (TCS) is a osmosensitive system where gene expression is modulated by sensing a change in intracellular osmolarity. Our composite part nicknamed "TCS basal" could be used an osmosensor mechanism where under high cellular osmolarity, green colour would be observed due to promoter OmpC regulated eGFP, while under low osmolarity, red colour will be observed due to promoter OmpF regulated mRFP1. This system was helpful for our biosensor where the target molecule (saxitoxin) induced significant osmotic changes within the cell. The Two Component System with reporter proteins formed a readout for this osmolarity changes. TCS is an attractive target for synthetic biologists as it is a modular, sensory signalling pathway. TCS could respond to environmental changes such as osmolarity, pH, temperature, phophor levels, etc. Teams working to identify both conditions possible such as high AND low pH, or high AND low osmolarity, could use our composite part which has red and green reporter proteins for effective response visualisation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1918
    Illegal AgeI site found at 2030
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None