Regulatory

Part:BBa_K3927005

Designed by: Chew Chin Wei   Group: iGEM21_NUS_Singapore   (2021-10-13)
Revision as of 13:54, 18 October 2021 by CWChew (Talk | contribs) (→‎Description)


PGK1-TetO

Native PGK1p promoter with TetO operator downstream of TATA box such that promoter is repressed when TetO is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

PGK1-tetO is a PGK1 S.cerevisiae promoter with a tet-operon inserted 20 basepairs downstream of it’s TATA box(Figure 1). TetR has been shown to bind to and repress tetO containing synthetic promoter in S.cerevisiae[1]. TetO was placed downstrem of the TATA box in order to construct a promoter that was constitutive unless TetO was expressed in the same cell

Usage

Design

Characterization

[edit]
Categories
Parameters
None