DNA

Part:BBa_K3805209

Designed by: Lijuan Wang   Group: iGEM21_BNU-China   (2021-09-30)
Revision as of 12:58, 18 October 2021 by Wangwang (Talk | contribs)


Bi-stable Switch

The bistable switch is composed of trc promoter(BBa_K3805208), P2 promoter(BBa_I746104), lacI- LVA (BBa_C0012)and mcherry-ompt.When AIP is released, it will act on P2 promoter and start the expression of lacI-LVA and downstream mcherry-ompt. LacI-LVA inhibits trc promoter.When the concentration of AIP decreases, the P2 promoter will be closed, and the inhibition of lacI-LVA on the trc promoter will be relieved.The trc promoter starts the expression of the target protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None