![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4088888
Designed by: Ruslan Vasiliev, Yaroslav Ugolkov, Alice Potapova, Pavel Ivanov-Rostovtsev Group: iGEM21_Moscow_City (2021-10-18)
dCas13a-Clact
С-terminal fragment of β-lactamase fused with dCas13.
β-Lactamase (AmpR) -
C-terminal fragment of β-lactamase -
dCAS13 is a catalytically dead Cas13a enzyme(formerly C2c2). The Cas13 family of enzymes includes at least 4 families, for example Cas13a, which is used in this biobrick. dCas13a interacts with gRNA about 65 nucleotides in length. dCas13a protein complexes with gRNA detect complementary RNA in the sample. Cas13 based detection is specific and can be customized to recognize single nucleotides on the target RNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1580
Illegal BglII site found at 1790
Illegal BglII site found at 2057
Illegal BglII site found at 2822
Illegal XhoI site found at 3751 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |