Composite

Part:BBa_K3868097

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 08:57, 18 October 2021 by Minter (Talk | contribs)


pCBE

pET--MME-eGFP plasmid contains pT7, lacO, MME, thrombin, eGFP-His-tag etc.Based on the plasmid of pET-24a, the C-terminal of MME was fused with eGFP in order to characterize the yield of MME. Moreover, in order to purify the MME , the enzyme loci of thrombin was inserted between MME and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-MME-eGFP. The fluorescence intensity of eGFP could be used to indicate the expression level of MME.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1572
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1572
    Illegal NheI site found at 1331
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1572
    Illegal BglII site found at 5013
    Illegal BamHI site found at 3610
    Illegal XhoI site found at 4616
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1572
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1572
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None