Regulatory

Part:BBa_K3927020

Designed by: Chew Chin Wei   Group: iGEM21_NUS_Singapore   (2021-10-13)
Revision as of 07:44, 18 October 2021 by CWChew (Talk | contribs) (→‎Characterization)


3C120-CYC-LacO-Flo1

3C120-CYC-LacO controlling of expression such that in the presence of blue light(dependent on EL222 expression) AND NOT LacI, yeast express the flocculation phenotype.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 203
    Illegal SpeI site found at 535
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 185
    Illegal SpeI site found at 535
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 203
    Illegal SpeI site found at 535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 203
    Illegal SpeI site found at 535
    Illegal AgeI site found at 439
    Illegal AgeI site found at 481
    Illegal AgeI site found at 616
    Illegal AgeI site found at 886
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

Design

Usage

Characterization

File:T--NUS Singapore--results tab5 4.png
Description here


[edit]
Categories
Parameters
None