Coding

Part:BBa_K3952000:Design

Designed by: B. Liam, D. Amanda, R. Jazmine, S. Brianna   Group: iGEM21_SUNY_Oneonta   (2021-09-30)
Revision as of 04:19, 18 October 2021 by Jazmine124 (Talk | contribs)


APOB-100 (Wild Type)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a positive control of the APOB gene allele that will be used within a Flappase genetic testing system.

A SpaI Restriction Site was found at 283. To adhere to RCF1000 assembly, a silent mutation was applied to the codon located within 285 and 287. The original codon was AGA and was altered to AGG.


Source

The source of the APOB-100 sequence is the genomic sequence from Bos taurus, apolipoprotein B genome. (GeneBank number: NC_037338.1)

References

https://pubmed.ncbi.nlm.nih.gov/26763170/