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Coding
Part:BBa_K3927006
Designed by: Chew Chin Wei Group: iGEM21_NUS_Singapore (2021-10-13)
yeLacI
LacI codon optimized for expression in S.cerevisiae
Description
LacI is a bacterial repressor commonly used in genetic circuits[1]. It has also been shown to be able to repress it’s cognate LacO sequence when expressed in S.cerevisiae[2]. The NUS iGEM 2021 team thus added a yeast optimized LacI to be able to control synthetic promoters in a repressible manner.
Usage
LacI can be used as a transregulatory element for promoters containing the Lac operon sequence downstream of the TATA box
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 253
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1044
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 253
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 253
Illegal NgoMIV site found at 595 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |