Composite
Part:BBa_K3941005:Design
Designed by: Can Bora Ciner Group: iGEM21_Saint_Joseph (2021-10-17)
pET29b+EGII
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 22
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 93
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 22
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 22
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
The EGII gene comes from T. reesei. We codon optimized the sequence and inserted gene in this composite part to express it.