Composite
SPIN

Part:BBa_K3776019

Designed by: Helene Heling Huang   Group: iGEM21_HK_SSC   (2021-10-14)
Revision as of 09:11, 16 October 2021 by Meowstarperson (Talk | contribs)


INTEGRATE system

Designed by Vo et al. (2020), this protein complex includes transposases TnsA, TnsB, TnsC and Cas enzymes Cas 8, Cas7, Cas6. These proteins are guided by a crRNA prefix flanked by two repeats. Users should clone their crRNA between these two repeats. This RNA guided protein complex inserts a donor DNA located between two flanks behind the into the genome of the host organism. Users should clone their genes of interest between these two flanks.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1578
    Illegal NheI site found at 3665
    Illegal NheI site found at 7783
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1004
    Illegal BglII site found at 1795
    Illegal BglII site found at 2325
    Illegal BglII site found at 7570
    Illegal BamHI site found at 147
    Illegal BamHI site found at 8698
    Illegal XhoI site found at 8681
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7702
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 113
    Illegal BsaI.rc site found at 87
    Illegal SapI.rc site found at 3081


[edit]
Categories
Parameters
None