Coding
cas8

Part:BBa_K3776013:Design

Designed by: Helene Heling Huang   Group: iGEM21_HK_SSC   (2021-10-01)
Revision as of 17:59, 17 October 2021 by Meowstarperson (Talk | contribs)


cas8


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 434
    Illegal BglII site found at 964
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1720


Design Notes

Source

References

Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration. Nature, 571(7764), 219–225. https://doi.org/10.1038/s41586-019-1323-z