Composite

Part:BBa_K3888011:Design

Designed by: Linhan Lei   Group: iGEM21_SJTang   (2021-10-01)
Revision as of 16:10, 1 October 2021 by Registry (Talk | contribs)

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[Ni-Fe] Hydrogenase Core gene HoxG1 and HoxK1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 363
    Illegal XbaI site found at 465
    Illegal SpeI site found at 531
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 531
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 678
    Illegal BamHI site found at 1593
    Illegal BamHI site found at 2932
    Illegal XhoI site found at 1915
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 363
    Illegal XbaI site found at 465
    Illegal SpeI site found at 531
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 363
    Illegal XbaI site found at 465
    Illegal SpeI site found at 531
    Illegal AgeI site found at 1809
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Change signal sequence of HoxK1


Source

Hydrogenovibrio marinus MH-110

References