Composite

Part:BBa_K3771015

Designed by: KAO, YI-CHING   Group: iGEM21_NCKU_Tainan   (2021-10-01)
Revision as of 11:31, 17 October 2021 by Ariel0119 (Talk | contribs)


PlacI-OmpA/OprF
Description

PlacI-ompA/oprF is part of the IFN-γ sensing system. lacI promoter regulates the constitutive expression of OmpA/OprF.

Biology

When there is no lac repressor, the lacI promoter facilitates the constitutive expression of OmpA/OprF. In the IFN-γ sensing system, binding of IFN-γ to the OmpA/OprF chimeric protein activates the pspA promoter, producing the enzyme required for the synthesis of taurine.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1039
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None