Part:BBa_K3771047

PkatG

Description

P_katG is the promoter of the katG gene. KatG protein has the function of bifunctional hydroperoxidase I (HPI) and plays an important role in oxidative stress response. The katG promoter (P_katG) is stimulated by oxidative stress, leading to the expression of the downstream gene. Besides, P_katG is activated by OxyR protein^[1].

Figure 1. Transcription unit of the katG gene in E. coli K-12^[2].

Usage and Biology

After conducting colony PCR from the E. coli MG1655, the P_katG fragment can be amplified from the chromosome and the experiment result can be checked by agarose gel electrophoresis. The result is shown in Figure 2.

Figure 2. The electrophoresis result of P_katG fragment from the chromosome PCR. M: Marker; Lane 1: katG promoter (P_katG) (217 bp).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 6
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]