Coding

Part:BBa_K3815009

Designed by: Hiroto Koga   Group: iGEM21_Kyoto   (2021-10-16)
Revision as of 09:42, 16 October 2021 by Registry (Talk | contribs)

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We used His tag to purify the peptides. The peptide His-tag fused was recovered by Ni chromatography, and DTT was added to cleave the N-terminus of the intein.Then, we can get the purpose protein. In this part, the purpose protein is NOP1.This is a small peptide that can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene and aging of the cut flowers.

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