Coding

Part:BBa_K3815004

Designed by: Shiotsu Koudai   Group: iGEM21_Kyoto   (2021-10-15)
Revision as of 08:59, 17 October 2021 by Shiotsu (Talk | contribs)


HIV-1 reverse transcriptase p51

This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein. In this part, the purpose protein is NOP1.This is a small peptide that can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene and aging of cut flowers. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 320
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 320
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 320
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 320
    Illegal AgeI site found at 966
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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