Coding

Part:BBa_K3815003

Designed by: Shiotsu Koudai   Group: iGEM21_Kyoto   (2021-10-15)
Revision as of 03:43, 16 October 2021 by Registry (Talk | contribs)

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B. stearothermophilus DNA polymerase

We used ELK16 system to purify the peptides. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the purpose protein. In this part, the purpose protein is LL37.This is antimicrobial peptide that can inhibit the both gram-negative bacteria and gram-positive bacteria. In our experiment, we can use it to inhibit the bacteria in the vase water.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 393
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1003
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None