Part:BBa_K4073000:Design

BBa_K407300 regulates salt tolerance in plants

This composite part consists of a CrGDPH3 salt-inducible promoter, a medium-strength RBS, the AtHKT1 gene, and a terminator. The regulation of the expression of the AtHKT1 gene is the function of this part. The CrGPDH3 salt-inducible promoter was used for the overexpression of proteins in a transgene in microalga Chlamydia reinhardtii. We then decided to utilize this promoter because of its high NaCl response elements. The RBS initiates translation of the HKT1 gene, which is essential for its protein synthesis. Finally, we used a composite terminator which signals the end of transcription in our part. This is essential, as if we did not utilize a terminator, the AtHKT1 gene could potentially be over-expressed, leading to decreased salt concentrations which could inhibit plant growth (salt is an essential nutrient used in plant growth).

The source of the medium strength RBS, HKT1 gene, and terminator all came from the iGEM registry. The RBS is the part labeled Bba_B0032, the HKT1 gene is the protein coding sequence of the part labeled "BBa_K2665007”, while the terminator is the part "BBa_B0015." The promoter was derived from the NCBI sequence for the CrGPDH3 salt-inducible promoter.