Part:BBa_K3832007
Toggle-switch circuit with GFP and RFP
This part is an updated version of the traditional toggle-switch developed by James J. Collins's group in 2000(Gardner et al. Nature, 2000), which contains two feedbacks each controls the other, and can achieve bistability of protein expression in different inducing conditions.Two promoter-repressor systems (lacUV5 promoter-LacI and lambda-CI857) with sfGFP and mRFP respectively, was constructed in our updated version, to monitor the two states of the circuit. With induction of IPTG, the downstream genes of lacUV5, that is, CI protein and mRFP will expressed, while those in the downstream of lambda promoter (LacI and sfGFP) will be repressed. Even without IPTG induction after several hours, the lack of LacI expression will result in the stability of red fluorescence. At temperatures above 42 degrees Celsius, gene expression will be flipped into another state, the stable expression of LacI and sfGFP, and the state will maintain even without heat. The GFP and RFP can be altered with other functional genes such as tyrptohan synthesitic genes to achieve the bistable expression and synthesis of tyrptohan.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2854
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2854
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2854
Illegal AgeI site found at 1891
Illegal AgeI site found at 3436
Illegal AgeI site found at 3548 - 1000COMPATIBLE WITH RFC[1000]
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