Generator

Part:BBa_K3962347:Design

Designed by: Siheng Li   Group: iGEM21_Leiden   (2021-10-12)
Revision as of 09:08, 12 October 2021 by Registry (Talk | contribs)

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J23113 promoter regulated constant expression of toxin CcdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 177


Design Notes

J23113 promoter has low transcriptional activity. Using it to regulate the toxin gene can not only prevent high lethality, but also maintain its function. For CcdB, it has also been used as a part of a kill switch. We used a PCR-based cloning method to clone the construct.



Source

All parts come from the iGEM registry.


References