Plasmid_Backbone
Part:BBa_K4047024
Designed by: An Nguyen Group: iGEM21_MiamiU_OH (2021-10-06)
pAM2991
Backbone sequence used for restriction digest assembly of our pF, pT, and pFT plasmids. This sequence contains the promoter, coding portion, and terminator for streptomycin/spectinomycin resistance (SmR). It also contains an origin of replication for the plasmid, a lacI promoter, LacI coding sequence for a lac repressor, lac operator, and a trc promoter.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 7968
Illegal XbaI site found at 2
Illegal XbaI site found at 2546
Illegal XbaI site found at 2860
Illegal PstI site found at 14
Illegal PstI site found at 2558
Illegal PstI site found at 5013
Illegal PstI site found at 5815 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 7968
Illegal NheI site found at 2673
Illegal PstI site found at 14
Illegal PstI site found at 2558
Illegal PstI site found at 5013
Illegal PstI site found at 5815
Illegal NotI site found at 515 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 7968 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 7968
Illegal XbaI site found at 2
Illegal XbaI site found at 2546
Illegal XbaI site found at 2860
Illegal PstI site found at 14
Illegal PstI site found at 2558
Illegal PstI site found at 5013
Illegal PstI site found at 5815 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 7968
Illegal XbaI site found at 2
Illegal XbaI site found at 2546
Illegal XbaI site found at 2860
Illegal PstI site found at 14
Illegal PstI site found at 2558
Illegal PstI site found at 5013
Illegal PstI site found at 5815
Illegal NgoMIV site found at 1043
Illegal NgoMIV site found at 3454 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 198
Illegal BsaI site found at 2068
Illegal BsaI.rc site found at 4839
Illegal BsaI.rc site found at 5605
Illegal SapI site found at 3756
[edit]
Categories
Parameters
//plasmidbackbone
None |