Composite

Part:BBa_K3832007

Designed by: Hongyu Chen   Group: iGEM21_XJTU-China   (2021-09-26)
Revision as of 15:31, 26 September 2021 by Registry (Talk | contribs)

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Toggle-switch circuit with GFP and RFP

This part is a kind of toggle-switch circuit, which can turn into bistable statement of protein expression in different inducing conditions. Two Promoter-Repressor gene systems (lacUV5 promoter-lacI and Plambda-CI857) are contained in this circuit. Once being induced by IPTG, the downstream genes of lacUV5 will expression stably, while those in the downstream of Plambda would be repressed. At temperatures above 42 degrees Celsius, gene expression moves into the opposite situation. With RFP CDS following the lacUV5 promoter and GFP CDS following the Plambda promoter, the effects of bistability can be more easily represented. Additional genes can be added after the GFP/RFP to achieve its bistable expression.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2854
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2854
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2854
    Illegal AgeI site found at 1891
    Illegal AgeI site found at 3436
    Illegal AgeI site found at 3548
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
biologyE.coli