Coding

Part:BBa_K3788023:Design

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)
Revision as of 20:24, 28 September 2021 by Registry (Talk | contribs)

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Expression of GFP and of an optimized design of the timer device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1568
    Illegal PstI site found at 1671
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2266
    Illegal PstI site found at 1568
    Illegal PstI site found at 1671
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2586
    Illegal BamHI site found at 2259
    Illegal XhoI site found at 2135
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1568
    Illegal PstI site found at 1671
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1568
    Illegal PstI site found at 1671
    Illegal NgoMIV site found at 2335
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 867
    Illegal SapI.rc site found at 2089


Design Notes

-


Source

sequence was given by LISM laboratory from CNRS. GFP coding sequence was taken corresponding to Part:BBa_E0040


References