Coding

Part:BBa_K3788000:Experience

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)
Revision as of 13:20, 15 October 2021 by Rpages (Talk | contribs)


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Applications of BBa_K3788000

PCR amplification of strepcyt1Aa gene :

The DNA sequence of strepcyt1Aa was amplify by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is 775 pb and a 800pb band is observed. Amplification of DNAs sequences with primers were validated.

T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png

Figure. Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning. a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 pB), flagcry11Aa (expected 1847 pB), strepcyt1Aa (expected 775 pB) and 6hisp20 (expected 568 pB); L (SMART Ladder, its size is shown on the right side).

User Reviews

UNIQ4d4f78ddf09dde40-partinfo-00000000-QINU UNIQ4d4f78ddf09dde40-partinfo-00000001-QINU