Part:BBa_K3788000

StrepII-Cyt1Aa

BBa_K3788000 sequence is coding for Cyt1Aa, a toxins from Bacillus thuringiensis. This toxins have a strep tag II in Nter to allow to purified the protein Cyt1Aa and allow the detection and specific identification of Cyt1Aa by WesterBlot. The gene cyt1Aa was optimised for E. coli K12.

strepII_cyt1Aa gene is 777pB Strep_Cyt1Aa protein is 28,5 kDa

Mode of action of Cyt toxins: Cyt (Cytolitic) toxins are produced in pro-toxin form, they will undergo proteolytic cleavage in N-ter and C-ter allowing the release of the active form of Cyt toxins. The mature forms will interact with the membrane lipids of the midgut cells and will insert themselves into the membrane, forming β-barrels. Cyt toxin are different in them domains.

Cyt1Aa would facilitate the binding of Cry11Aa to the BBMV receptor, by interaction of the loop α8, β4 and loop 2 regions of Cry11Aa at the level of the loop β6 – αE and part of β7 of Cyt1Aa. This interaction allows easy insertion of Cry11Aa into membranes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]