Part:BBa_K4081994

GAP promoter

Generally, GAP promoter can drive the expression of glyceraldehyde-3-phosphate dehydrogenase gene in many species.

Biology and Usage

A lot of attention has been drawn to Glyceraldehyde-3-Phosphate Dehydrogenase (GAP) promoter. GAP mRNA accounts for 2–5% of the poly(A) RNA in yeasts, suggesting that it can be effectively used in the high-efficiency expression of transgenes[1]. In our project, we use GAP promoter to promote the expression of FKP. General vectors have been designed to rely on homologous GAPDH promoter regions to drive the expressions of heterologous genes in yeasts and other filamentous fungi.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design and Properties We use GAP promoter to promote the expression of FKP. We tested the GAP promoter. We transform the gene "GAP promoter and FKP (https://parts.igem.org/Part:BBa_K4081992# BBa_K4081992])" into Saccharomyces cerevisiae BY4741 by lithium acetate conversion method to integrate the genes into the genome of BY4741 for expression.

Experimental approach

Reference

[1]Jia Y, Li S, Allen G, Feng S, Xue L. A novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter for expressing transgenes in the halotolerant alga Dunaliella salina. Curr Microbiol. 2012 May;64(5):506-13.