Reporter

Part:BBa_E0050

Designed by: Austin Che and Reshma Shetty   Group: iGEM07_Example   (2007-07-23)
Revision as of 19:03, 2 March 2009 by Austin (Talk | contribs)

GFP.lacZalpha fusion

This part is a fusion protein between GFP (BBa_E0040) and the N-terminal alpha fragment of lacZ (which encodes β-galactosidase). It is a reporter which can be measured via fluorescence from GFP or through a Miller assay of β-galactosidase.

The GFP does not function well. See E0051 for a better part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
Parameters
None