Composite

Part:BBa_K3941004

Designed by: Can Bora Ciner   Group: iGEM21_Saint_Joseph   (2021-10-13)
Revision as of 19:52, 13 October 2021 by Boraciner (Talk | contribs)


pET29b+CelAB

BBa_K3941004 is a sequence that we used in our expression plasmids (pET29b). We produce CelAB via this specific sequence.


This composite part is made out of 4 different parts: - T7 Promoter (BBa_K3941000) - LacO (BBa_K1624002) - CelAB (BBa_K3941000) - T7 Terminator


800px-T--Saint_Joseph--Diagram-CelAB-Whole.png


Figure 1: T7 promoter, Lac Operon, Codon optimized CelAB, 6XHis and terminator


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 51
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 930
    Illegal XhoI site found at 972
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 51
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None