Coding

Part:BBa_K3853012

Designed by: Peng Luo   Group: iGEM21_CPU_CHINA   (2021-09-24)
Revision as of 17:41, 13 October 2021 by Lp-tiffany (Talk | contribs)


sfGFP Codon adapted for Pichia pastoris

This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). This part of the amino acid sequence is the same as BBa_I746916. Using the Thermo GeneArt online tool geneoptimize, we optimize the codon of the protein gene sequence to improve its expression in Pichia pastoris. We use No part name specified with partinfo tag. to construct the expression system and to express and to purify the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None