Regulatory

Part:BBa_K3930006

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-07)
Revision as of 21:05, 7 October 2021 by Registry (Talk | contribs)

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Inducer Z3EV of the estradiol promoter

This sequence aload the production of the activator Z3eV, which can activate the promoter Z3eV upon binding to estradiol. This sequence must be used when a plasmidic construction uses the promoter Z3eV from part (BBa_K3930007)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
    Illegal AgeI site found at 1527
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1849
    Illegal BsaI.rc site found at 987


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Categories
Parameters
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