Coding

Part:BBa_K3982001

Designed by: Akankshya Ramkrishna Sahu   Group: iGEM21_IISER_Berhampur   (2021-10-01)
Revision as of 15:44, 12 October 2021 by Akankshya16 (Talk | contribs)


Cas14a1 expression gene

This sequence encodes the Cas14a1 protein. It is a highly compact RNA guided nuclease used by Team IISER_Berhampur 2021 in Project CODE M.

Usage and Biology

Cas14a1 (also called Un1Cas12f1) is a miniature cas protein used in CRISPR/Cas technology identified from archaebacteria and bacteriophages. It is a RNA guided nuclease that cleaves ssDNA without any Protospacer Adjacent Motif (PAM) specificity i.e. PAM independent ssDNA cleavage. This enables high-fidelity Single-nucleotide polymorphism (SNP) genotyping. It is guided by a single guide RNA (sgRNA) which has two components - CRISPR RNA (crRNA) and Trans-activating crispr RNA (tracrRNA). 

Click here to know more about how this part is used in project CODE M by Team IISER_Berhampur 2021.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1357
    Illegal BamHI site found at 951
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
    Illegal AgeI site found at 607
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None