Part:BBa_K3930032

Spectinomycine selective cassette

This cassette allows the selection of Cyanobacteria transformant upon addition of spectinomycin into the media of culture. The sequence of the gene carrying the resistance is codon optimized for an expression into Synechcoccus elongatus.

Introduction

The SpecR cassette is composed by the promoter PaadA and the resistance gene specR (aadA). The resistance gene is based on the part (BBa_BBa_K1323012), but it was codon optimized for expression into S.elongatus.

This gene codes for a Aminoglycoside adenylyltransferase , which inactivate the spectinomycin by adding a adenyl group on it. Therefore, this cassette aload the selection of Yeast transformant upon addition of spectinomycin into the media of culture.

Part characterization

The part (BBa_K3930032) was used to select the right genomic integration of the part (BBa_K3930026). Transformants were plated onto BG11 + 10 μg.ml-1 spectinomycin Petri dish. The Toulouse INSA UPS team manage to select a transformant with this selection marker (for more details, check the (BBa_K3930026) part page)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1032
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 7