Composite

Part:BBa_K3982006

Designed by: Akankshya Ramkrishna Sahu   Group: iGEM21_IISER_Berhampur   (2021-10-01)
Revision as of 15:13, 11 October 2021 by Akankshya16 (Talk | contribs)


6xHis - Tagged Cas14a1 with TEV site

Cas14a1 is a type V-F exceptionally compact RNA-guided nuclease. Cas14 forms a complex with RNA-guided nuclease and it can cleave ssDNA without PAM specificity. It comes tagged with 6xHis and has the Tobacco Etch Virus (TEV) protease and cleavage site for easy purification. In our project CODE M, Cas14a1 and sgRNA complex is used to detect SNPs in the Mycobacterium tuberculosis genome in a sputum sample. Upon detection, it triggers cleavage of a Fluorescence-Quencher pair ssDNA reporter and thus fluorescence is emitted which confirms the presence of SNPs in the genome

6xHis tagged Cas14a1 gene with TEV site











Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 201
    Illegal PstI site found at 367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 201
    Illegal PstI site found at 367
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1411
    Illegal BamHI site found at 1005
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 201
    Illegal PstI site found at 367
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 201
    Illegal PstI site found at 367
    Illegal AgeI site found at 661
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None