RNA

Part:BBa_K3982004:Design

Designed by: Akankshya Ramkrishna Sahu   Group: iGEM21_IISER_Berhampur   (2021-10-01)
Revision as of 11:55, 11 October 2021 by Akankshya16 (Talk | contribs) (→‎References)


CODE M sgRNA for targeting wildtype katG gene in Mycobacterium tuberculosis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The scaffold region has been designed as referred from Ref. 1. It consists of the tracrRNA and regions of crRNA. The guiding region is the 20 bp spacer sequence which is complementary to the katG gene in Mtb and referred from Ref 2.

Source

References

1) Kim, D.Y., Lee, J.M., Moon, S.B. et al. Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus. Nat Biotechnol (2021). https://doi.org/10.1038/s41587-021-01009-z NCBI Reference Sequence for Mycobacterium tuberculosis H37Rv: NC_000962.3

2) Musser, J. M., Kapur, V., Williams, D. L., Kreiswirth, B. N., van Soolingen, D., & van Embden, J. D. (1996). Characterization of the catalase-peroxidase gene (katG) and inhA locus in isoniazid-resistant and -susceptible strains of Mycobacterium tuberculosis by automated DNA sequencing: restricted array of mutations associated with drug resistance. The Journal of infectious diseases, 173(1), 196–202. https://doi.org/10.1093/infdis/173.1.196