Composite
Part:BBa_K3739106
Designed by: Shichen Geng Group: iGEM21_XMU-China (2021-09-30)
K525998(T7-RBS)-Aly01-his-CBM-hutH-B0010
Aly01 is a signal peptide from the alginate lyase of V.natriegens, his-tag enable us to purify the linking protein, CBM can bind to cellulose and hutH is a histidine transaminase which converts histidine into urocanic acid
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 653
Illegal NgoMIV site found at 1089
Illegal NgoMIV site found at 1824
Illegal NgoMIV site found at 2060
Illegal AgeI site found at 111
Illegal AgeI site found at 399
Illegal AgeI site found at 489
Illegal AgeI site found at 726
Illegal AgeI site found at 1553
Illegal AgeI site found at 2049 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |