DNA

Part:BBa_K3791011

Designed by: Laura Sánchez Ruiz, Auba Fuster Palŕ   Group: iGEM21_UPF_Barcelona   (2021-10-10)
Revision as of 11:52, 10 October 2021 by Registry (Talk | contribs)

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gRNA Chloramphenicol construct

This construct corresponds to the full DNA sequence that allows for the transcription of the guide RNA targeting chloramphenicol resistance. For that purpose, in addition to the gRNA, it includes a promoter. Particularly, it is the T7 promoter, which used in pertinent competent cells is indirectly inducible by the addition of IPTG (as the T7 RNA-polymerase needs IPTG to be expressed) and therefore permits transcriptional modulation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/crispr-cas/diagnosis
//function/crispr/grna/construct
Parameters
None