Coding

Part:BBa_K3930026

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-08)
Revision as of 13:03, 8 October 2021 by Registry (Talk | contribs)

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3,6-nonadienal induction system and expression in S. elongatus (pCONCOMBRE)

pCONCOMBRE is a pAM4951 based vector designed to carry: - the Nb-9-LOX (BBa_K3930030), Cm-9-HPL (BBa_K3930031) and LacI genes (Part:BBa_C0012), for the production of 3,6-nonadienal and LacI repressor. - the inducible promoters Trc-theoE-riboswitch (BBa_K3930029), driving the expression of Nb-9-LOX and Cm-9-HPL. - the resistance marker SpecR (BBa_K3930032) to select for Cyanobacteria integrants


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2244
    Illegal EcoRI site found at 2250
    Illegal XbaI site found at 590
    Illegal XbaI site found at 6286
    Illegal XbaI site found at 8003
    Illegal XbaI site found at 8030
    Illegal PstI site found at 8476
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2244
    Illegal EcoRI site found at 2250
    Illegal NheI site found at 777
    Illegal NheI site found at 872
    Illegal NheI site found at 899
    Illegal PstI site found at 8476
    Illegal NotI site found at 7951
    Illegal NotI site found at 7959
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2244
    Illegal EcoRI site found at 2250
    Illegal BglII site found at 6283
    Illegal BglII site found at 6289
    Illegal BamHI site found at 3717
    Illegal XhoI site found at 3753
    Illegal XhoI site found at 4736
    Illegal XhoI site found at 6361
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2244
    Illegal EcoRI site found at 2250
    Illegal XbaI site found at 590
    Illegal XbaI site found at 6286
    Illegal XbaI site found at 8003
    Illegal XbaI site found at 8030
    Illegal PstI site found at 8476
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2244
    Illegal EcoRI site found at 2250
    Illegal XbaI site found at 590
    Illegal XbaI site found at 6286
    Illegal XbaI site found at 8003
    Illegal XbaI site found at 8030
    Illegal PstI site found at 8476
    Illegal NgoMIV site found at 1936
    Illegal AgeI site found at 2163
    Illegal AgeI site found at 2190
    Illegal AgeI site found at 4955
    Illegal AgeI site found at 7844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8686
    Illegal BsaI.rc site found at 911
    Illegal SapI site found at 2653
    Illegal SapI site found at 3728
    Illegal SapI.rc site found at 7355


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