Coding
Part:BBa_K808027
Designed by: Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling Group: iGEM12_TU_Darmstadt (2012-09-02)
EstA : inactive fusion protein for bacterial surface expression
EstA is a membrane bound but inactive esterase from Pseudomonas aeruginosa. It can be used (unlike https://parts.igem.org/Part:BBa_K633001 BBa_K633001) for the cell surface expression of enzymes. Similiar to OmpA it allows to perform bacterial display of e.g. hydrolases like it has already been shown by Becker, Theile, Heppeler & Michalczyk, 2005. We used EstA to create our chimeric protein BBa_K808030 (Figure 1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 97
Illegal NgoMIV site found at 235
Illegal NgoMIV site found at 775
Illegal NgoMIV site found at 1144 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//cds/membrane
//cds/membrane/extracellular
//cds/membrane/extracellular
protein |