Part:BBa_K3838222
J-BSH
Bilesalthydrolase (BSH), responsible for hydrolysis in combination with bile salts to produce free bile acids and amino acids. BSH activity in gastrointestinal tract was mainly from Firmicutes (30%), Bacteroidetes (14.4%) and actinobacteria (8.9%). Impaired microbial enzyme activity in IBD patients leads to impaired bile acid metabolism, characterized by the inability to hydrolyze, transform, and desulphurize bile salts. Microbial BSH activity removes glycine or taurine molecular coupling to produce unbound bile acids. Abnormal intestinal bile salt metabolism enhances the inflammatory response of the intestinal epithelium, which worsens IBD. It has been documented that the ingestion of probiotics with BSH activity can promote the bioconversion of intestinal bile salts, thereby alleviating the inflammatory response induced by high doses of intestinal bile salts. Therefore, we selected heterologous expression of BSH gene from Lactobacillus acidophilus. In a team project, the BSH-related parts are structured as follows: J23100 promoter +RBS+BSH+T7 terminator
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 969
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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