Composite

Part:BBa_K3755029:Design

Designed by: Qi Xin   Group: iGEM21_ShanghaiTech_China   (2021-10-01)
Revision as of 09:05, 9 October 2021 by HeteroauXin (Talk | contribs) (→‎Design Notes)


cfa-c-mfp5 CDS-his tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 355
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a complete CDS for 3*repeat Mfp5 protein expression and it use be uesd with part:BBa_K3755030. However, the start codon and stop codon is not contained in the sequence. So please add the start codon and the stop codon to the beginning and the end of the sequence before you put this part into the protein expression plasmid(which we recommend pET28).

Source

unwritten

References