Temporary

Part:BBa_K3726080:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-08)
Revision as of 19:19, 8 October 2021 by Registry (Talk | contribs)

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P_PrbcL-m36


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 225
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 225
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 225
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.



Source

The promoter sequence corresponds with a modified version of the native Synechococcus PCC11801 phycocyanin β-1 subunit promoter.

References