Coding

Part:BBa_K3853003

Designed by: Gongyu Liu   Group: iGEM21_CPU_CHINA   (2021-09-20)
Revision as of 14:58, 20 September 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


dCas9

A Cas9 protein with H840A and D10A mutations has only nucleic acid binding activity, but lacks nucleic acid cleavage activity

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3960
    Illegal AgeI site found at 3304
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None