Terminator

Part:BBa_K3726095:Design

Designed by: Lucia Tapia   Group: iGEM21_MADRID_UCM   (2021-10-06)
Revision as of 10:59, 6 October 2021 by LuciaTapia (Talk | contribs)

T(5a)_Teck


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.

This terminator part spans the 5 position of the MoClo syntax. The downstream overhang is CGCT. This part behaves like a normal phytobrick standard terminator which in the design of Marburg Collection is connected to a 3’ Connector part during the level 1 MoClo reaction.

Source

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References

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