Part:BBa_K3970000
AmpR
1.Usage and Biology
Ampicillin resistance genes are the most widely used selective markers in gene manipulation, and most plasmid vectors cloned in E. coli carry this gene. Penicillin inhibits the synthesis of cell wall peptidoglycan, binds to and inhibits the activity of related enzymes, and inhibits the peptide transfer reaction. The ampicillin resistance gene encodes an enzyme that is secreted into the periplasmic region of the bacteria, inhibiting the peptide transfer reaction and catalyzing β -lactam ring hydrolysis, thereby detoxicating ampicillin.Penicillin is a general term for a class of compounds, its molecular structure is composed of side chain R-CO- and main nuclear 6-amino penicilanic acid two parts. There is A saturated thiazole ring (A) and A β -lactam ring in 6-APA+.6-APA is A dipeptide formed by the condensation of L-hemi-dephosphoric acid and valine.
2.sequence
Atgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaa
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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